HPLC Simulatorthe free, open-source HPLC simulator

HPLC simulation
and teaching resources

Browser-based tools for studying high-performance liquid chromatography. Calculate chromatograms, draw flow paths, and examine how operating conditions affect a separation.

Open HPLC SimulatorEducational resources
HPLC Simulator gradient-elution chromatogram with numbered peaks, a red selected-compound trace, and increasing solvent B on the right axis.

Applications

Both applications run in the browser without Java.

0246810Time (min)Detector response
Chromatographic separations

HPLC Simulator

Calculate chromatograms for isocratic and gradient runs. Adjust mobile-phase composition, flow rate, column properties, and sample composition to compare retention times, peak widths, and resolution.

Open HPLC Simulator
4 mil ID · 10 cm · 0.811 µL4 mil ID · 10 cm · 0.811 µL4 mil ID · 61.7 cm · 5 µL4 mil ID · 10 cm · 0.811 µL4 mil ID · 10 cm · 0.811 µL4 mil ID · 10 cm · 0.811 µL4 mil ID · 10 cm · 0.811 µLHPLC pumpHPLC pump6 port, 2 pos valve — double-click to switch positionPosition 1 / 26 port, 2 pos valveAutosamplerAutosamplerWasteWasteColumn4.6 × 150 mmColumnDetectorDetectorWasteWasteConnected tubing — HPLC pump, port 1Connected tubing — 6 port, 2 pos valve, port 11Connected tubing — 6 port, 2 pos valve, port 22Connected tubing — 6 port, 2 pos valve, port 33Connected tubing — 6 port, 2 pos valve, port 44Connected tubing — 6 port, 2 pos valve, port 55Connected tubing — 6 port, 2 pos valve, port 66Connected tubing — Autosampler, port 1Connected tubing — Waste, port 1Connected tubing — Column, port 1Connected tubing — Column, port 2Connected tubing — Detector, port 1Connected tubing — Detector, port 2Connected tubing — Waste, port 1
System plumbing

HPLC Fluid Visualizer

Draw flow paths using pumps, valves, tubing, columns, and detectors. Calculate system backpressure, band broadening, and gradient delay for the connected components.

Open HPLC Fluid Visualizer

Educational resources

Download contributed problem sets, simulator exercises, instructor notes, and a companion spreadsheet.

View resources